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mouse anti bag1  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse anti bag1
    Mouse Anti Bag1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bag+1/Bag-1+Antibody/pmc12973372-3-0-4
    Average 93 stars, based on 120 article reviews
    mouse anti bag1 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Western Blot:

    Article Title: Development of Bag-1L as a therapeutic target in androgen receptor-dependent prostate cancer
    Article Snippet: .. Western blotting was carried out using standard protocols with following antibodies: Bag-1 (FL-274 and F-7, Santa Cruz Biotechnology, Dallas, TX or Y166, Abcam, Cambridge, UK), Bag-1L (was obtained from Andrew Cato; Crocoll et al., 2000), AR (N-20, Santa-Cruz Biotechnology), Hsp70 (K-20, Santa Cruz Biotechnology), Hsp70/Hsc70 (W27, Santa Cruz Biotechnology), Flag-tag (M2, Sigma Aldrich, St. Louis, MO), HA-tag (F-7, Santa Cruz Biotechnology or Abcam), b-actin (C4, Santa Cruz Biotechnology or Abcam), vinculin (V9131, Sigma Aldrich), Lamin B1 (EPR8985, Abcam). .. Bag-1L and AR inhibitors were produced by Jakob Troppmair (Thio-2), Xavier Salvatella (EPI-001 and iodo-EPI-002) or purchased from Selleckchem (enzalutamide).

    Transfection:

    Article Title: Bag-1 stimulates Bad phosphorylation through activation of Akt and Raf kinases to mediate cell survival in breast cancer.
    Article Snippet: .. 50–70% confluent cells in 6-well plates were transfected with Bag-1 expression plasmid using X-tremeGENE HP DNA transfection reagent (Roche) or with siRNAs using HiPerFect transfection reagent (Qiagen), according to the manufacturers’ protocols. siRNAs were used to silence the expression of Bag-1 (sc-29,211, Santa Cruz), Raf-1 (sc-29,462, Santa Cruz), and B-Raf (#8935, Cell Signaling Technology). .. Negative control siRNAs (Qiagen) were also used.

    Expressing:

    Article Title: Bag-1 stimulates Bad phosphorylation through activation of Akt and Raf kinases to mediate cell survival in breast cancer.
    Article Snippet: .. 50–70% confluent cells in 6-well plates were transfected with Bag-1 expression plasmid using X-tremeGENE HP DNA transfection reagent (Roche) or with siRNAs using HiPerFect transfection reagent (Qiagen), according to the manufacturers’ protocols. siRNAs were used to silence the expression of Bag-1 (sc-29,211, Santa Cruz), Raf-1 (sc-29,462, Santa Cruz), and B-Raf (#8935, Cell Signaling Technology). .. Negative control siRNAs (Qiagen) were also used.

    Plasmid Preparation:

    Article Title: Bag-1 stimulates Bad phosphorylation through activation of Akt and Raf kinases to mediate cell survival in breast cancer.
    Article Snippet: .. 50–70% confluent cells in 6-well plates were transfected with Bag-1 expression plasmid using X-tremeGENE HP DNA transfection reagent (Roche) or with siRNAs using HiPerFect transfection reagent (Qiagen), according to the manufacturers’ protocols. siRNAs were used to silence the expression of Bag-1 (sc-29,211, Santa Cruz), Raf-1 (sc-29,462, Santa Cruz), and B-Raf (#8935, Cell Signaling Technology). .. Negative control siRNAs (Qiagen) were also used.

    Membrane:

    Article Title: A rationally designed self-immolative linker enhances the synergism between a polymer-rock inhibitor conjugate and neural progenitor cells in the treatment of spinal cord injury.
    Article Snippet: .. The membrane was blocked with 1 % BSA in Tris buffer saline (TBS) with 0.1 % Tween-20 for 1 h at room temperature and incubated at 4 ◦C overnight with the following primary antibody solutions: pAKT (1:1000 4060, Cell Signaling), Bag-1 (1:1000 sc-376848, Santa Cruz), Bcl-2 (1:1000 2870S, Cell Signaling), BDNF (1:500 sc-655114, Santa Cruz), cAMP (1:500 ab24851, Abcam), caspase-3 (1:1000 9662S Cell Signaling), p-MYPT (1:1000 ABS45 Millipore), and p-mTOR (1:1000 2971 Cell Signaling). .. ; AMPK (1:1000 2535 Cell Signaling), LC3I (1:1000 3868 Cell Signaling).

    Saline:

    Article Title: A rationally designed self-immolative linker enhances the synergism between a polymer-rock inhibitor conjugate and neural progenitor cells in the treatment of spinal cord injury.
    Article Snippet: .. The membrane was blocked with 1 % BSA in Tris buffer saline (TBS) with 0.1 % Tween-20 for 1 h at room temperature and incubated at 4 ◦C overnight with the following primary antibody solutions: pAKT (1:1000 4060, Cell Signaling), Bag-1 (1:1000 sc-376848, Santa Cruz), Bcl-2 (1:1000 2870S, Cell Signaling), BDNF (1:500 sc-655114, Santa Cruz), cAMP (1:500 ab24851, Abcam), caspase-3 (1:1000 9662S Cell Signaling), p-MYPT (1:1000 ABS45 Millipore), and p-mTOR (1:1000 2971 Cell Signaling). .. ; AMPK (1:1000 2535 Cell Signaling), LC3I (1:1000 3868 Cell Signaling).

    Incubation:

    Article Title: A rationally designed self-immolative linker enhances the synergism between a polymer-rock inhibitor conjugate and neural progenitor cells in the treatment of spinal cord injury.
    Article Snippet: .. The membrane was blocked with 1 % BSA in Tris buffer saline (TBS) with 0.1 % Tween-20 for 1 h at room temperature and incubated at 4 ◦C overnight with the following primary antibody solutions: pAKT (1:1000 4060, Cell Signaling), Bag-1 (1:1000 sc-376848, Santa Cruz), Bcl-2 (1:1000 2870S, Cell Signaling), BDNF (1:500 sc-655114, Santa Cruz), cAMP (1:500 ab24851, Abcam), caspase-3 (1:1000 9662S Cell Signaling), p-MYPT (1:1000 ABS45 Millipore), and p-mTOR (1:1000 2971 Cell Signaling). .. ; AMPK (1:1000 2535 Cell Signaling), LC3I (1:1000 3868 Cell Signaling).

    Article Title: Inhibition of nitric oxide production of activated mice peritoneal macrophages is independent of the Toxoplasma gondii strain
    Article Snippet: .. Cells were incubated for 1 h in the serum of mice chronically infected with T. gondii diluted 1:5000 in PBS-BSA and rabbit polyclonal antibodies that recognise BAG-1 (kindly provided by Dr Louis Weiss), iNOS (M 19, Santa Cruz Biotechnology), phosphorylated Smad2 (P-SMAD2) (# 3101, Cell Signaling Technology) or LC3A (L8793, Sigma), all diluted 1:200 in PBS-bovine serum albumin (PBS-BSA). ..

    other:

    Article Title: Fisetin induces apoptosis through mitochondrial apoptosis pathway in human uveal melanoma cells.
    Article Snippet: Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan Department of Ophthalmology, Sijhih Cathay General Hospital, New Taipei City, Taiwan Department of Ophthalmology, Cathay General Hospital, Taipei, Taiwan Tissue Culture Center, New York Eye and Ear Infirmary of Mount Sinai, Icahn School of Medicine at Mount Sinai, New York, New York Department of Ophthalmology, New York Eye and Ear Infirmary of Mount Sinai, Icahn School of Medicine at Mount Sinai, New York, New York Department of Optometry, Asia University, Taichung, Taiwan Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan Institute of Biochemistry, Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan

    Infection:

    Article Title: Inhibition of nitric oxide production of activated mice peritoneal macrophages is independent of the Toxoplasma gondii strain
    Article Snippet: .. Cells were incubated for 1 h in the serum of mice chronically infected with T. gondii diluted 1:5000 in PBS-BSA and rabbit polyclonal antibodies that recognise BAG-1 (kindly provided by Dr Louis Weiss), iNOS (M 19, Santa Cruz Biotechnology), phosphorylated Smad2 (P-SMAD2) (# 3101, Cell Signaling Technology) or LC3A (L8793, Sigma), all diluted 1:200 in PBS-bovine serum albumin (PBS-BSA). ..



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